{"id":365,"date":"2016-11-17T09:43:47","date_gmt":"2016-11-17T08:43:47","guid":{"rendered":"https:\/\/www.biogalenys.com\/phototoxicite"},"modified":"2020-01-29T06:06:20","modified_gmt":"2020-01-29T05:06:20","slug":"phototoxicity","status":"publish","type":"page","link":"https:\/\/www.biogalenys.com\/en\/in-vitro-toxicology\/phototoxicity","title":{"rendered":"phototoxicity"},"content":{"rendered":"
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IN VITRO TOXICOLOGY \u2013 PHOTOTOXICITY<\/h1> <\/div>\n<\/div>\n\n\n
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The Biogalenys laboratory offers in-vitro phototoxicity testing in line with OCDE guideline n\u00b0432.<\/p>

The in-vitro phototoxicity test 3T3 NRU relies on comparing a chemical substance\u2019s cytotoxicity with and without exposure to a dose of non-cytotoxic simulated solar light. The test is based on measuring the cellular viability of BALB\/c 3T3 cells, evaluated by reduced uptake of a vital dye, neutral red, by cells. Cell viability is measured 24 hours after exposure.<\/p>

The test is based on the PIF\u00a0model (Photo-irritancy Factor):<\/p>

This model is based on the comparison of 2 obtained EC50<\/sub> values, for cytotoxicity (-UV) and phototoxicity (+UV) and makes it possible to quantify the photo-irritancy factor (PIF).<\/p>

If it is not possible to quantify IC50, then the substance has no PIF.<\/p>

Si PIF < 2: No phototoxicity.<\/p>

Si 2 < PIF < 5: Probable phototoxicity.<\/p>

Si PIF > 5: Proven phototoxicity.<\/p> <\/div>\n<\/div>\n\n\t <\/div>\n\t \t<\/div>\n\t\t <\/div>\n\t \n\t<\/div>\n\t\n\t\t\n\t

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Application<\/h2>