TRANSMEMBRANE – TRANSCUTANEOUS PERMEATION
The Biogalenys laboratory carries out transcutaneous permeation or non-permeation studies in line with the OECD 428 guidelines.
Thanks to its expertise, Biogalenys supports you to put in place the appropriate protocols for your research programmes.
Transcutaneous permeation studies are carried out using Franz cell membranes with either fresh or frozen human skin samples (0 – 24h or 0 – 16h or kinetic study).
The analytical methods used (HPLC-UV-DAD, HPLC-MS-MS, GC-MS, etc.) are developed and validated within our laboratories.
Estimates are achieved within the different layers of skin (stratum corneum, epidermis, dermis) and (receptor fluid).
Depending on the galenic formulation, we determine the composition’s influence on the cutaneous absorption rate (solvent, co-solvent, accelerator, excipient, active ingredient concentrations).
In order to compare permeation rates in terms of differences in composition, and to measure flux, the studies can be carried out using the following types of filter membranes:
- Hydrophobic (Millipore FGLP HVHP, MAGNA Nylon, FGLP, PTFE)
- Hydrophilic (GH-Polypro, polyamide, anapore)